|
KMID : 1140120100150030190
|
|
Cancer Prevention Research
2010 Volume.15 No. 3 p.190 ~ p.197
|
|
|
Morin Inhibits the Growth of Murine Hepatoma Cells via Cell Cycle Arrest and Induction of Apoptosis
|
|
Kim Mi-Na
Ahn Eun-Young
Park Sang-Eun
Hossain Mohammad Akbar
Kim Min-Young
Moon Jeon-Ok
Kim Nam-Deuk
Yoon Jeong-Hyun
|
|
|
Abstract
|
|
|
|
Morin (3,5,7,2’,4’-pentahydroxyflavone) is a common dietary flavonoid that exhibits various biological activities, including anti-proliferative, anti-tumor promotion, anti-oxidative, and anti-inflammatory effects. In the present study, we investigated the effects of morin on the cell growth and the viabilities of hepa1c1c7 murine hepatoma cells. Flow cytometric analysis showed that morin-treated cells increased sub-G1 peak accompanied by DNA cleavage and phosphatidylserine externalization, indicating early apoptosis. Flow cytometric analysis also revealed that morin treatment induced G2/M arrest in the cell cycle progression. In addition, morin treatment induced alterations in expression levels of proteins which are linked to cell cycle and apoptosis, such as cyclin B1, Cdc2, Fas, Fas ligand, Bax, Bcl-2, caspase-9, caspase-3, and poly (ADP-ribose) polymerase, in a concentration-dependent manner. Taken together, these results suggest that morin inhibit cell growth and induce cytotoxicity of murine hepatoma cells through G2/M phase arrest and apoptosis.
|
|
|
KEYWORD
|
|
|
Morin, Cell cycle, G2/M phase, Apoptosis, Hepatoma cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|